Comparison of Ear Notch Immunohistochemistry, Ear Notch Antigen-Capture ELISA, and Buffy Coat Virus Isolation for Detection of Calves Persistently Infected with Bovine Viral Diarrhea Virus
نویسندگان
چکیده
منابع مشابه
Comparison of ear notch immunohistochemistry, ear notch antigen-capture ELISA, and buffy coat virus isolation for detection of calves persistently infected with bovine viral diarrhea virus.
Two techniques performed on skin biopsy samples (ear notches), immunohistochemistry (IHC) and antigen-capture ELISA (AgELISA), were compared for detection of bovine viral diarrhea virus (BVDV) persistent infection (PI) in 559 Angus calves between the ages of 1 and 5 months. The calves also were tested for BVDV infection using virus isolation (VI) and reverse transcription (RT)-PCR on buffy coat...
متن کاملcomparison of ear notch immunofluorescent, buffy coat antigen-capture elisa and rt-pcr for detection of calves suspected to acutely infected with bovine pesti virus
towelve calves of 4-10 months old with clinical signs suspected to bovine viral diarrhea virus (bvdv) infection were selected for this study. histopathologic sections were performed on formalin fixed paraffin-embded ear notch biopsies, mounted on poly-l-lysine coated slides and stained for bvdv by anti-bvdv monoclonal antibody labelled with fluorescein isothiocyanate. stained sections were exam...
متن کاملImplementation of immunohistochemistry on frozen ear notch tissue samples in diagnosis of bovine viral diarrhea virus in persistently infected cattle
BACKGROUND Bovine viral diarrhea is a contagious disease of domestic and wild ruminants and one of the most economically important diseases in cattle. Bovine viral diarrhea virus belongs to the genus Pestivirus, within the family Flaviviridae. The identification and elimination of the persistently infected animals from herds is the initial step in the control and eradication programs. It is the...
متن کاملMolecular characterization and phylogenetic analysis of bovine viral diarrhea virus in dairy herds of Fars province, Iran
Bovine viral diarrhea virus (BVDV) is one of the most important viral pathogens of cattle worldwide. The aim of present study was to determine the molecular characterization and phylogenetic analysis of BVDV infection in dairy herds of Fars province, Iran. For initial screening, a total of 400 blood samples were collected from 12 industrial dairy herds with previous history of diarrhea, abortio...
متن کاملGeneration of calves persistently infected with HoBi-like pestivirus and comparison of methods for detection of these persistent infections.
The identification and elimination of persistently infected (PI) cattle are the most effective measures for controlling bovine pestiviruses, including bovine viral diarrhea virus (BVDV) and the emerging HoBi-like viruses. Here, colostrum-deprived calves persistently infected with HoBi-like pestivirus (HoBi-like PI calves) were generated and sampled (serum, buffy coat, and ear notches) on the da...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2005
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063870501700203